Arrestin-1 engineering facilitates complex stabilization with native rhodopsin
نویسندگان
چکیده
منابع مشابه
Enhanced Arrestin Facilitates Recovery and Protects Rods Lacking Rhodopsin Phosphorylation
G protein-coupled receptors (GPCRs) are the largest family of signaling proteins expressed in every cell in the body and are targeted by the majority of clinically used drugs [1]. GPCR signaling, including rhodopsin-driven phototransduction, is terminated by receptor phosphorylation followed by arrestin binding [2]. Genetic defects in receptor phosphorylation and excessive signaling by overacti...
متن کاملDynamics of arrestin-rhodopsin interactions: acidic phospholipids enable binding of arrestin to purified rhodopsin in detergent.
We report that acidic phospholipids can restore the binding of visual arrestin to purified rhodopsin solubilized in n-dodecyl-beta-d-maltopyranoside. We used this finding to investigate the interplay between arrestin binding and the status of the retinal chromophore ligand in the receptor binding pocket. Our results showed that arrestin can interact with the late photoproduct Meta III and conve...
متن کاملEach rhodopsin molecule binds its own arrestin.
Arrestins (Arrs) are ubiquitous regulators of the most numerous family of signaling proteins, G protein-coupled receptors. Two models of the Arr-receptor interaction have been proposed: the binding of one Arr to an individual receptor or to two receptors in a dimer. To determine the binding stoichiometry in vivo, we used rod photoreceptors where rhodopsin (Rh) and Arr are expressed at comparabl...
متن کاملRegulation of rhodopsin dephosphorylation by arrestin.
We have characterized the opsin phosphatase activities in extracts of rod outer segments and determined their relationship to known protein phosphatases. The opsin phosphatase activity in the extracts was not due to protein phosphatases 1, 2B, or 2C because it was neither stimulated by Mg2+ or Ca2+/calmodulin nor inhibited by protein phosphatase inhibitors-1 or -2. Opsin phosphatase activity in...
متن کاملArrestin–GRK1 Competition Affects Rhodopsin Active Time
Phototransduction begins when a photon activates a rhodopsin molecule. Activated rhodopsin activates the G-protein transducin, which activates phosphodiesterase, leading to decreased cGMP concentration and closure of cyclic-nucleotide-gated channels. Termination of this process requires inactivation of rhodopsin and transducin. The former is mediated by arrestin, which separates the bleached ch...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Scientific Reports
سال: 2019
ISSN: 2045-2322
DOI: 10.1038/s41598-018-36881-4